Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs

作者：Chun‐Hua Xing, Shun Chen, Yue Wang, Zhen‐Nan Pan, Yuanjing Zou, Shao‐Chen Sun, Zili Ren, Yu Zhang · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2022 · DOI：10.1186/s40104-022-00732-0 · 被引用次数：27 · 研究领域：Pesticide and Herbicide Environmental Studies、Reproductive Biology and Fertility、Weed Control and Herbicide Applications

Abstract Background Recently, defects in mammalian oocytes maturation induced by environmental pollution results in the decreasing animal reproduction. Animal exposed to glyphosate is largely unavoidable because glyphosate is one of the most widely used herbicide worldwide due to its high-efficiency and broad-spectrum effects, which causes glyphosate an environmental contaminant found in soil, water and food. During the last few years, the growing and wider use of glyphosate has raised great concerns about its effects of reproductive toxicity. In this study, using porcine models, we investigated effects of glyphosate on organelle functions during oocyte meiosis. Results The results showed glyphosate exposure disrupted porcine oocyte maturation. Expression levels of cumulus expansion-related genes were interfered, further indicating the meiotic defects. The damaging effects were mediated by destruction of mitochondrial distribution and functions, which induced ROS accumulation and oxidative stress, also indicated by the decreased mRNA expression of related antioxidant enzyme genes. We also found an interference of endoplasmic reticulum (ER) distribution, disturbance of Ca 2+ homeostasis, as well as fluctuation of ER stress, showing with the reduced ER stress-related mRNA or protein expression, which could indicate the dysfunction of ER for protein processing and signal transduction in glyphosate-exposed oocytes. Moreover, glyphosate exposure induced the disruption of lysosome ...