Genome-Wide Characterization of QYYZ-Like PRRSV During 2018–2021

作者：Hu Xu, Lirun Xiang, Yan‐Dong Tang, Chao Li, Jing Zhao, Bangjun Gong, Qi Sun, Chaoliang Leng, Jinmei Peng, Qian Wang, Guohui Zhou, Tongqing An, Xuehui Cai, Zhi‐Jun Tian, Hongliang Zhang, Mingxin Song · 发表于：Frontiers in Veterinary Science · 年份：2022 · DOI：10.3389/fvets.2022.945381 · 被引用次数：20 · 研究领域：Animal Virus Infections Studies、Virus-based gene therapy research、Viral gastroenteritis research and epidemiology

In the last decade, the emergence of QYYZ-like porcine reproductive and respiratory syndrome virus (PRRSV) has attracted increasing attention due to the high incidence of PRRSV mutation and recombination. However, the endemic status and genomic characteristics of the QYYZ-like strains are unclear. From 2018 to October 2021, 24 QYYZ-like PRRSV isolates were obtained from 787 PRRSV-positive clinical samples. Only one QYYZ-like positive sample was from a northern province, and the rest were from central and southern provinces. We selected 9 samples for whole-genome sequencing, revealing genome lengths of 15,008–15,316 nt. We retrieved all the available whole-genome sequences of QYYZ-like PRRSVs isolated in China from 2010 to 2021 ( n = 28) from GenBank and analyzed them together with the new whole-genome sequences ( n = 9). Phylogenetic tree analysis based on the ORF5 gene showed that all QYYZ-like PRRSV strains belonged to sublineage 3.5 but were clustered into three lineages (sublineage 1.8, sublineage 3.5, and sublineage 8.7) based on whole-genome sequences. Genomic sequence alignment showed that QYYZ-like strains, have characteristic amino acids insertions or deletions in the Nsp2 region (same as NADC30, JXA1 and QYYZ) and that thirteen strains also had additional amino acid deletions, mostly between 468 and 518 aa. Moreover, QYYZ-like strains (sublineage 3.5) have seven identical characteristic amino acid mutations in ORF5. Recombination analysis revealed that almost all QY...