Boronate Affinity-Based Electrochemical Aptasensor for Point-of-Care Glycoprotein Detection

作者：Qiong Hu, Shuhan Hu, Shiqi Li, Sijie Liu, Yiyi Liang, Xiaojing Cao, Yilin Luo, Wanjing Xu, Haocheng Wang, Jianwen Wan, Wenxing Feng, Li Niu · 发表于：Analytical Chemistry · 年份：2022 · DOI：10.1021/acs.analchem.2c01699 · 被引用次数：47 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、Peptidase Inhibition and Analysis

As a class of oligosaccharide chain-containing proteins, glycoproteins are of great value in screening and early diagnosis of malignant tumors and other major diseases. Herein, we report a universal boronate affinity-based electrochemical aptasensor for point-of-care glycoprotein detection. Aptasensing of glycoproteins involves the specific recognition and capture of target glycoproteins by end-tethered nucleic acid aptamers and the site-specific labeling of ferrocene tags via the phenylboronic acid (PBA)-based boronate affinity interactions because the cis -diol sites of oligosaccharide chains on glycoproteins can selectively react with the PBA receptor groups to form cyclic phenylborates in aqueous basic media. Due to the presence of hundreds to thousands of cis -diol sites on a glycoprotein, a large number of ferrocene tags can be recruited for the signal-on aptasensing of glycoproteins at a low-abundance level, eliminating the need for extra amplification strategies. As a result, the boronate affinity-based electrochemical aptasensor is highly sensitive and selective for glycoprotein detection and tolerant to the false-positive results. The detection limit for α-fetoprotein (AFP) is 0.037 ng/mL, with a linear response ranging from 0.1 to 100 ng/mL. In addition to the merits of simple operation, short assay time, and low detection cost, the aptasensor is applicable to the detection of glycoproteins in serum samples and the point-of-care detection using disposable flexible ...