Comprehensive evaluation of caloric restriction-induced changes in the metabolome profile of mice

作者：Dadi Xie, Jinxi Huang, Qiang Zhang, Shiyuan Zhao, Hongjia Xue, Qingqing Yu, Zhuohao Sun, Jing Li, Xiumei Yang, Minglei Shao, Deshui Pang, Pei Jiang · 发表于：Nutrition & Metabolism · 年份：2022 · DOI：10.1186/s12986-022-00674-4 · 被引用次数：15 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、Dietary Effects on Health、Adipose Tissue and Metabolism

OBJECTS: Caloric restriction (CR) is known to extend lifespan and exert a protective effect on organs, and is thus a low-cost and easily implemented approach to the health maintenance. However, there have been no studies that have systematically evaluated the metabolic changes that occur in the main tissues affected by CR. This study aimed to explore the target tissues metabolomic profile in CR mice. METHODS: Male C57BL/6J mice were randomly allocated to the CR group (n = 7) and control group (n = 7). A non-targeted gas chromatography-mass spectrometry approach and multivariate analysis were used to identify metabolites in the main tissues (serum, heart, liver, kidney, cortex, hippocampus, lung, muscle, and white adipose) in model of CR. RESULTS: We identified 10 metabolites in the heart that showed differential abundance between the 2 groups, along with 9 in kidney, 6 in liver, 6 in lung, 6 in white adipose, 4 in hippocampus, 4 in serum, 3 in cortex, and 2 in muscle. The most significantly altered metabolites were amino acids (AAs) (glycine, aspartic acid, L-isoleucine, L-proline, L-aspartic acid, L-serine, L-hydroxyproline, L-alanine, L-valine, L-threonine, L-glutamic acid, and L-phenylalanine) and fatty acids (FAs) (palmitic acid, 1-monopalmitin, glycerol monostearate, docosahexaenoic acid, 16-octadecenoic acid, oleic acid, stearic acid, and hexanoic acid). These metabolites were associated with 7 different functional pathways related to the metabolism of AAs, lipids, and ...