Effects of Acute Hypoxic Stress on Physiological and Hepatic Metabolic Responses of Triploid Rainbow Trout (Oncorhynchus mykiss)

作者：Buying Han, Yuqiong Meng, Haining Tian, Changzhong Li, Yaopeng Li, Caidan Gongbao, Wenyan Fan, Rui Ma · 发表于：Frontiers in Physiology · 年份：2022 · DOI：10.3389/fphys.2022.921709 · 被引用次数：51 · 研究领域：Physiological and biochemical adaptations、Aquaculture disease management and microbiota、Aquaculture Nutrition and Growth

This experiment simulated the hypoxic environment caused by actual production operations in fish farming (i.e., catching, gathering, transferring, and weighting) to study the effects of acute hypoxic conditions on the physiological and metabolic responses of triploid rainbow trout ( O. mykiss ). Two groups of fish weighting 590 g were sampled in the normoxia group (dissolved oxygen above 7 mg/L) and hypoxia group (dissolved oxygen ranged from 2 to 5 mg/L for 10 min). The results showed that 1) regarding stress response, hypoxia increased plasma levels of cortisol, heat shock protein 70 (HSP-70), lysozyme, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatine phosphokinase (CPK); induced the expression of hepatic genes encoding nuclear factor erythroid 2 related factor 2 ( Nrf2 ), interferon γ ( IFN-γ ) and interleukin-1β ( IL-1β ). 2) Regarding metabolism response, hypoxia increased plasma levels of globulin (GLOB), glucose (GLU), triglyceride (TG) and lactate dehydrogenase (LDH); upregulated the hepatic gene expression of phosphoenolpyruvate carboxykinase, ( PEPCK ), pyruvate dehydrogenase kinase ( PDK1 ), acetyl-CoA carboxylase ( ACC ) and acetyl-CoA oxidase ( ACO ); downregulated the hepatic gene expression of carnitine palmitoyl transferase 1 ( CPT1 ); and unchanged the expression of hepatic genes in glycolysis and autophagy. 3) In response to hypoxia-inducible factors (HIFs), the hepatic HIF-2α gene was activated in the hypoxia group, but HIF-1α g...