Characterization and Functional Studies of a Novel Depolymerase Against K19-Type Klebsiella pneumoniae

作者：Yunfen Hua, Yongqin Wu, Minjie Guo, Rui‐Jing Ma, Qingchuan Li, Qingchuan Li, Zheyuan Hu, Hongrui Chen, Xingyu Zhang, Hui Li, Qingtian Li, Qingtian Li, Ping He · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.878800 · 被引用次数：27 · 研究领域：Antibiotic Resistance in Bacteria、Pneumocystis jirovecii pneumonia detection and treatment、Cancer therapeutics and mechanisms

Carbapenem-resistant Klebsiella pneumoniae (CRKP), a pathogen that causes severe nosocomial infections and yields a high mortality rate, poses a serious threat to global public health due to its high antimicrobial resistance. Bacteriophages encode polysaccharide-degrading enzymes referred to as depolymerases that cleave the capsular polysaccharide (CPS), one of the main virulence factors of K. pneumoniae . In this study, we identified and characterized a new capsule depolymerase K19-Dpo41 from K. pneumoniae bacteriophage SH-KP156570. Our characterization of K19-Dpo41 demonstrated that this depolymerase showed specific activities against K19-type K. pneumoniae . K19-Dpo41-mediated treatments promoted the sensitivity of a multidrug-resistant K19-type K. pneumoniae strain to the bactericidal effect of human serum and significantly increased the survival rate of Galleria mellonella infected with K19-type K. pneumoniae . Our results provided strong primary evidence that K19-Dpo41 was not only effective in capsular typing of K19-type K. pneumoniae but promising in terms of developing new alternative therapeutic strategies against K19-type CRKP infections in the future.