Water Rewarming After Seawater Hypothermia Mitigates IL-1β in Both Intestinal Tissue and Blood

作者：Dandan Li, Wei Ma, Ming Xiong, Peng Xie, Youxin Feng, Dongdong Liu, Yuanyuan Qiao, Chenghe Shi · 发表于：Therapeutic Hypothermia and Temperature Management · 年份：2022 · DOI：10.1089/ther.2021.0033 · 被引用次数：3 · 研究领域：Thermal Regulation in Medicine、Respiratory Support and Mechanisms、Thermoregulation and physiological responses

In this study, the rat models of severe hypothermia induced by seawater immersion were established in artificial seawater immersion at 15°C for 5 hours. With the rewarming measurement of 37°C water bath, the rewarming effects were evaluated by monitoring basic vital signs and dynamically detecting intestinal inflammation cytokines. Fifty Sprague-Dawley rats were randomly divided into five groups including the control group (group C), hypothermia group (group H), 2-hour rewarming group (group R2), 6-hour rewarming group (group R6), and 12-hour rewarming group (group R12), with 10 in each group. The basic vital signs of rats (i.e., core temperature, respiration, heart rate, and muscle tremor) were constantly recorded. The inflammatory factors were detected in the intestinal tissue via a protein chip GSR-CAA-67 of Innopsys, and the verification by reverse transcription-quantitative polymerase chain reaction. The levels of cytokines (interleukin IL-1β, IL-6, and IL-10) were detected from blood samples collected at the end of the observation period via enzyme-linked immunosorbent assay. The expression landscape of IL-1β in the intestinal tissue was validated by immunohistochemistry. Five hours of immersion in artificial seawater at 15°C successfully induced severe hypothermia of rats. After 2 hours of constant water bath rewarming at 37°C, the basic vital signs recovered to the normal level and maintained stably as well as the acute inflammatory reaction alleviated effectively, wh...