Inhibition of a Chromatin and Transcription Modulator, SLTM, Increases HIV-1 Reactivation Identified by a CRISPR Inhibition Screen

作者：Savannah F. Pedersen, Jack A. Collora, Rachel N. Kim, Kerui Yang, Anya Razmi, Allison A. Catalano, Yang-Hui Jimmy Yeh, Karam Mounzer, Pablo Tebas, Luis J. Montaner, Ya‐Chi Ho · 发表于：Journal of Virology · 年份：2022 · DOI：10.1128/jvi.00577-22 · 被引用次数：18 · 研究领域：HIV Research and Treatment、CRISPR and Genetic Engineering、Cytomegalovirus and herpesvirus research

HIV-1-infected cells, which can survive drug treatment and immune cell killing, prevent an HIV-1 cure. Immune recognition of infected cells requires HIV-1 protein expression; however, HIV-1 protein expression is limited in infected cells after long-term therapy. The ways in which the HIV-1 provirus is blocked from producing protein are unknown. We identified a new host protein that regulates HIV-1 gene expression. We also provided a new method of studying HIV-1-host factor interactions in cells from infected individuals. These improvements may enable future strategies to reactivate HIV-1 in infected individuals so that infected cells can be killed by immune cells, drug treatment, or the virus itself.