Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology

作者：Sagar Khavnekar, Veronika Vrbovská, Magda Zaoralová, Ron Kelley, Florian Beck, Sven Klumpe, Abhay Kotecha, Jürgen M. Plitzko, Philipp S. Erdmann · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2022 · DOI：10.1101/2022.06.16.496417 · 被引用次数：15 · 研究领域：Advanced Electron Microscopy Techniques and Applications、RNA and protein synthesis mechanisms、Force Microscopy Techniques and Applications

Abstract We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide evidence for the physical background of this improvement and demonstrate its usefulness by producing sub-5Å ribosome averages from yeast.