Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon

作者：Tarek Hilal, Benjamin Y. Killam, Milica Grozdanović, Malgorzata Dobosz-Bartoszek, J. Loerke, Jörg Bürger, Thorsten Mielke, Paul R. Copeland, Miljan Simonović, C.M.T. Spahn · 发表于：Science · 年份：2022 · DOI：10.1126/science.abg3875 · 被引用次数：71 · 研究领域：RNA modifications and cancer、RNA and protein synthesis mechanisms、Trace Elements in Health

The elongation of eukaryotic selenoproteins relies on a poorly understood process of interpreting in-frame UGA stop codons as selenocysteine (Sec). We used cryo-electron microscopy to visualize Sec UGA recoding in mammals. A complex between the noncoding Sec-insertion sequence (SECIS), SECIS-binding protein 2 (SBP2), and 40 S ribosomal subunit enables Sec-specific elongation factor eEFSec to deliver Sec. eEFSec and SBP2 do not interact directly but rather deploy their carboxyl-terminal domains to engage with the opposite ends of the SECIS. By using its Lys-rich and carboxyl-terminal segments, the ribosomal protein eS31 simultaneously interacts with Sec-specific transfer RNA (tRNA Sec ) and SBP2, which further stabilizes the assembly. eEFSec is indiscriminate toward l -serine and facilitates its misincorporation at Sec UGA codons. Our results support a fundamentally distinct mechanism of Sec UGA recoding in eukaryotes from that in bacteria.