Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

作者：Robert J. Gruninger, Xiu Min Zhang, M. L. Smith, L. Kung, D. Vyas, S. M. McGinn, Maik Kindermann, Min Wang, Zhi Liang Tan, K. A. Beauchemin · 发表于：Animal Microbiome · 年份：2022 · DOI：10.1186/s42523-022-00179-8 · 被引用次数：52 · 研究领域：Ruminant Nutrition and Digestive Physiology、Agriculture Sustainability and Environmental Impact、Reproductive Physiology in Livestock

Abstract Background The major greenhouse gas from ruminants is enteric methane (CH 4 ) which in 2010, was estimated at 2.1 Gt of CO 2 equivalent, accounting for 4.3% of global anthropogenic greenhouse gas emissions. There are extensive efforts being made around the world to develop CH 4 mitigating inhibitors that specifically target rumen methanogens with the ultimate goal of reducing the environmental footprint of ruminant livestock production. This study examined the individual and combined effects of supplementing a high-forage diet (90% barley silage) fed to beef cattle with the investigational CH 4 inhibitor 3-nitrooxypropanol (3-NOP) and canola oil (OIL) on the rumen microbial community in relation to enteric CH 4 emissions and ruminal fermentation. Results 3-NOP and OIL individually reduced enteric CH 4 yield (g/kg dry matter intake) by 28.2% and 24.0%, respectively, and the effects were additive when used in combination (51.3% reduction). 3-NOP increased H 2 emissions 37-fold, while co-administering 3-NOP and OIL increased H 2 in the rumen 20-fold relative to the control diet. The inclusion of 3-NOP or OIL significantly reduced the diversity of the rumen microbiome. 3-NOP resulted in targeted changes in the microbiome decreasing the relative abundance of Methanobrevibacter and increasing the relative abundance of Bacteroidetes . The inclusion of OIL resulted in substantial changes to the microbial community that were associated with changes in ruminal volatile fatty a...