Marine Natural Product Antimycin A Suppresses Wheat Blast Disease Caused by Magnaporthe oryzae Triticum

作者：Sanjoy Paul, Moutoshi Chakraborty, Mahfuzur Rahman, Dipali Rani Gupta, Nur Uddin Mahmud, Abdullah Al Mahbub Rahat, Aniruddha Sarker, Md. Abdul Hannan, Md Mahbubur Rahman, Abdul Mannan Akanda, Jalal Ahmed, Tofazzal Islam · 发表于：Journal of Fungi · 年份：2022 · DOI：10.3390/jof8060618 · 被引用次数：28 · 研究领域：Plant Pathogens and Fungal Diseases、Plant-Microbe Interactions and Immunity、Fungal Biology and Applications

The application of chemical pesticides to protect agricultural crops from pests and diseases is discouraged due to their harmful effects on humans and the environment. Therefore, alternative approaches for crop protection through microbial or microbe-originated pesticides have been gaining momentum. Wheat blast is a destructive fungal disease caused by the Magnaporthe oryzae Triticum (MoT) pathotype, which poses a serious threat to global food security. Screening of secondary metabolites against MoT revealed that antimycin A isolated from a marine Streptomyces sp. had a significant inhibitory effect on mycelial growth in vitro. This study aimed to investigate the inhibitory effects of antimycin A on some critical life stages of MoT and evaluate the efficacy of wheat blast disease control using this natural product. A bioassay indicated that antimycin A suppressed mycelial growth (62.90%), conidiogenesis (100%), germination of conidia (42%), and the formation of appressoria in the germinated conidia (100%) of MoT at a 10 µg/mL concentration. Antimycin A suppressed MoT in a dose-dependent manner with a minimum inhibitory concentration of 0.005 μg/disk. If germinated, antimycin A induced abnormal germ tubes (4.8%) and suppressed the formation of appressoria. Interestingly, the application of antimycin A significantly suppressed wheat blast disease in both the seedling (100%) and heading stages (76.33%) of wheat at a 10 µg/mL concentration, supporting the results from in vitro st...