Manufacturing Mesenchymal Stromal Cells for the Treatment of Osteoarthritis in Canine Patients: Challenges and Recommendations

作者：Ana Ivanovska, Mengyu Wang, Tarlan Eslami Arshaghi, Georgina Shaw, Joel M. Alves, Andrew W. Byrne, Steven Butterworth, Russell Chandler, Laura C. Cuddy, J. Dunne, Shane Guerin, R Moon Harry, Aidan McAlindan, Ronan A. Mullins, Frank Barry · 发表于：Frontiers in Veterinary Science · 年份：2022 · DOI：10.3389/fvets.2022.897150 · 被引用次数：32 · 研究领域：Mesenchymal stem cell research、Bone fractures and treatments、Tissue Engineering and Regenerative Medicine

characterization (mostly following protocols developed for human application), experimental testing in controlled studies and clinical use in veterinary patients. The ability of MSCs to interact with the inflammatory environment through immunomodulatory and paracrine mechanisms makes them a good candidate for treatment of inflammatory musculoskeletal conditions in canine species. Analysis of existing data shows promising results in the treatment of canine hip dysplasia, osteoarthritis and rupture of the cranial cruciate ligament in both sport and companion animals. Despite the absence of clear regulatory frameworks for veterinary advanced therapy medicinal products, there has been an increase in the number of commercial cell-based products that are available for clinical applications, and currently the commercial use of veterinary MSC products has outpaced basic research on characterization of the cell product. In the absence of quality standards for MSCs for use in canine patients, their safety, clinical efficacy and production standards are uncertain, leading to a risk of poor product consistency. To deliver high-quality MSC products for veterinary use in the future, there are critical issues that need to be addressed. By translating standards and strategies applied in human MSC manufacturing to products for veterinary use, in a collaborative effort between stem cell scientists and veterinary researchers and surgeons, we hope to facilitate the development of quality standar...