Hybrid system with stable structure of hard/soft tissue substitutes induces re‐osseointegration in a rat model of biofilm‐mediated peri‐implantitis

作者：Lianyi Xu, Qin Xu, Mahmood S. Mozaffari, Di Yan, Xiaojuan Sun, Yingguang Cao · 发表于：Journal of Biomedical Materials Research Part B Applied Biomaterials · 年份：2022 · DOI：10.1002/jbm.b.35102 · 被引用次数：5 · 研究领域：Bone Tissue Engineering Materials、Dental Implant Techniques and Outcomes、Orthopedic Infections and Treatments

Re-osseointegration of an infected/contaminated dental implant poses major clinical challenges. We tested the hypothesis that the application of an antibiotic-releasing construct, combined with hard/soft tissue replacement, increases the efficacy of reconstructive therapy. We initially fabricated semi-flexible hybrid constructs of β-TCP/PHBHHx, with tetracycline (TC) (TC amounts: 5%, 10%, and 15%). Thereafter, using in vitro assays, TC release profile, attachment to rat bone marrow-derived stem cells (rBMSCs) and their viability as well as anti-bacterial activity were determined. Thereafter, regenerative efficacies of the three hybrid constructs were assessed in a rat model of peri-implantitis induced by Aggregatibacter actinomycetemcomitans biofilm; control animals received β-TCP/Bio-Gide and TC injection. Eight weeks later, maxillae were obtained for radiological, histological, and histomorphometric analyses of peri-implant tissues. Sulcus bleeding index was chronologically recorded. Serum cytokines levels of IL-6 and IL-1β were also evaluated by enzyme-linked immunosorbent assay. Substantial amounts of tetracycline, from hybrid constructs, were released for 2 weeks. The medium containing the released tetracycline did not affect the adhesion or viability of rBMSCs; however, it inhibited the proliferation of A. actinomycetemcomitans. Osteogenesis and osseointegration were more marked for the 15% hybrid construct group than the other two groups. The height of attachment and i...