A protocol for applying a population-specific reference genome assembly to population genetics and medical studies

作者：Lian Deng, Bo Xie, Yimin Wang, Xiaoxi Zhang, Shuhua Xu · 发表于：STAR Protocols · 年份：2022 · DOI：10.1016/j.xpro.2022.101440 · 被引用次数：7 · 研究领域：Genomics and Phylogenetic Studies、Genomics and Rare Diseases、Genetic Associations and Epidemiology

With a growing number of available de novo sequenced genomes, protocols for their applications to population genetics will benefit our understanding of the human genome. Here we detail analytic steps to apply an example de novo reference genome to map and detect variants of short-read sequences from corresponding populations and to discover variants of disease-relevant genes. Using this protocol, we can improve variant discovery, better investigate population-specific genome properties, and evaluate the potential of sequenced genomes in medical studies. For complete details on the use and execution of this protocol, please refer to Lou et al. (2022).