Best practice standards for circular RNA research

作者：A. Nielsen, Albrecht Bindereif, Irene Bozzoni, Mor Hanan, Thomas B. Hansen, Manuel Irimia, Sebastián Kadener, Lasse S. Kristensen, Ivano Legnini, Mariangela Morlando, Morten T. Jarlstad Olesen, R. Jeroen Pasterkamp, Stephan Preibisch, Nikolaus Rajewsky, Christin Suenkel, Jørgen Kjems · 发表于：Nature Methods · 年份：2022 · DOI：10.1038/s41592-022-01487-2 · 被引用次数：217 · 研究领域：Circular RNAs in diseases、MicroRNA in disease regulation、RNA modifications and cancer

Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors' experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.