Antibacterial Effects of Commiphora gileadensis Methanolic Extract on Wound Healing

作者：Ayman Alhazmi, Abdullah F. Aldairi, Ahmad Al-Ghamdi, Anas M. Alomery, Abdulrahman Mujalli, Ahmad A. Obaid, Wesam F. Farrash, Mamdouh Allahyani, Ibrahim F. Halawani, Abdulelah Aljuaid, Sarah A. Alharbi, Mazen Almehmadi, Moodi S. Alharbi, Anmar A. Khan, Maisam.A. Jastaniah, Abdulrhman Alghamdi · 发表于：Molecules · 年份：2022 · DOI：10.3390/molecules27103320 · 被引用次数：32 · 研究领域：Pharmacological Effects of Medicinal Plants、Wound Healing and Treatments、Dermatological diseases and infestations

Commiphora gileadensis (CG) is a small tree distributed throughout the Middle East. It was traditionally used in perfumes in countries in this area. In Saudi Arabia, it was used to treat wounds burns and as an antidote to scorpion stings. This study aimed to evaluate the antimicrobial activity and cutaneous wound healing efficiency of the CG extracts using microbiological tests, rate of wound contraction and histopathological changes. CG plant were extracted using the methanol extraction technique; then, the methanolic extract was characterized using liquid chromatography coupled with mass spectrometry (LC−MS). Afterwards, a six-millimetre (mm) excision wound was induced in 60 male Balb/c mice. Mice were classified into two classes; each class consisted of three groups of 10 mice. In the non-infected wound class, the group I was assigned as control and received normal saline. Group II received gentamicin treatment, and group III treated with CG-methanolic extract. In the Staphylococcus aureus-infected class, group IV received normal saline, and groups V and VI were treated with gentamicin and CG-methanolic extract, respectively. The colonization of infected wounds was determined using colony-forming units (CFUs), and the percentage of wound contraction was measured in all groups. Finally, the histopathologic semi-quantitative determination of wound healing was evaluated by inflammatory cell infiltration, the presence of collagen fibres and granulation tissue, and the grade of...