[Retracted] Chinese Herb Jiedu Huayu Granules Inhibiting Immune and Inflammatory Response of Rats with Acute Liver Failure by Regulating the NF‐κB Signaling Pathway

作者：Wenjie Bai, Qinglan Shi, Jinyu Wu, Kejing Wang, Yueqiao Chen, Xiaocong Ma, Dewen Mao · 发表于：BioMed Research International · 年份：2022 · DOI：10.1155/2022/4479885 · 被引用次数：2 · 研究领域：Pharmacological Effects of Natural Compounds、Drug-Induced Hepatotoxicity and Protection、Ginger and Zingiberaceae research

Objective . To research the influence of Chinese medicine Jiedu Huayu granules (JDHY) on the immune response and inflammatory response of rats with acute liver failure (ALF) and investigate its related mechanism. Methods . Rats were randomly divided into 4 groups: control group ( n = 6) were injected with the same amount of normal saline; ALF group ( n = 10) were injected intraperitoneally with D‐GaIN (700 mg/kg) and LPS (10 μ g/kg); ALF+JDHY group ( n = 10) were given JDHY 57.55 g/kg/d by gavage for 7 days and injected intraperitoneally with D‐GaIN/LPS after the last dose; and ALF+BAY group ( n = 10) were given BAY 10 mg/kg/d by gavage for 7 days and injected intraperitoneally with D‐GaIN/LPS after the last dose. Changes in liver function and coagulation function were examined in rat serum; the pathological varieties of liver tissues were verified by HE staining; immunohistochemistry was utilized to determine the ratio of PCNA and F4/80 in liver tissues; the flow cytometry was applied to determine the ratio of CD4+/CD8+ cells in peripheral blood mononuclear cells (PBMCs); ELISA and qRT‐PCR were utilized to check the level of IL‐10, IL‐6, IL‐13, IL‐1 β , TNF‐ α , IFN‐ γ , and CD163 in serum and liver cells. Western blot was adopted to check the expression of apoptotic protein and expression and NF‐ κ B pathway‐related protein expression. Results . JDHY and BAY could decline the expression of AST, ALT, ALP, and TBiL in ALF rat serum significantly ( P < 0.01), increase PTA a...