A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens

作者：Yueqiu Li, Xiaoli Zhou, Xianjiao Zhang, Zhiqiang Xu, Honghong Dong, Guohui Yu, Ping Cheng, Qing Yao, Honghui Zhu · 发表于：AMB Express · 年份：2022 · DOI：10.1186/s13568-022-01393-y · 被引用次数：16 · 研究领域：Studies on Chitinases and Chitosanases、Enzyme Production and Characterization、Genomics and Phylogenetic Studies

Myxobacteria, as predatory bacteria, have good application potential in the biocontrol of pathogenic microorganisms. Extracellular enzymes are thought to play an important role in their predation and also provide resources for discovering new antibacterial molecules. We previously isolated a myxobacterium, Corallococcus silvisoli c25j21 GDMCC 1.1387, which is predatory to plant pathogenic bacteria. In this study, we identified an endolysin-like GH19 glycoside hydrolase, C25GH19B, from the genome of c25j21. After its heterologous expression and purification from E. coli, the enzymatic properties of C25GH19B were characterized. C25GH19B showed lysozyme activity with the optimal reaction conditions at 40 °C and pH 4.5-5.0. Moreover, C25GH19B showed bacteriolytic activity against both Gram-positive and Gram-negative plant pathogenic bacteria. Our research provides not only a candidate enzyme for the development of novel biocontrol agents but also an experimental basis for further study on the function and mechanisms of extracellular enzymes in myxobacterial predation.