Preterminal host dendritic cells in irradiated mice prime CD8+ T cell–mediated acute graft-versus-host disease

作者：Yi Zhang, Jean‐Pierre Louboutin, Jiang Zhu, Adam J. Rivera, Stephen G. Emerson · 发表于：Journal of Clinical Investigation · 年份：2002 · DOI：10.1172/jci0214989 · 被引用次数：177 · 研究领域：Hematopoietic Stem Cell Transplantation、Immunotherapy and Immune Responses、T-cell and B-cell Immunology

To understand the relationship between host antigen-presenting cells (APCs) and donor T cells in initiating graft-versus-host disease (GVHD), we followed the fate of host dendritic cells (DCs) in irradiated C57BL/6 (B6) recipient mice and the interaction of these cells with minor histocompatibility antigen-(miHA-) mismatched CD8 + T cells from C3H.SW donors.Host CD11c + DCs were rapidly activated and aggregated in the T cell areas of the spleen within 6 hours of lethal irradiation.By 5 days after irradiation, <1% of host DCs were detectable, but the activated donor CD8 + T cells had already undergone as many as seven divisions.Thus, proliferation of donor CD8 + T cells preceded the disappearance of host DCs.When C3H.SW donor CD8 + T cells were primed in vivo in irradiated B6 mice or ex vivo by host CD11c + DCs for 24-36 hours, they were able to proliferate and differentiate into IFN-γ-producing cells in β 2 -microglobulin-deficient (β 2 m -/-) B6 recipients and to mediate acute GVHD in β 2 m -/-→ B6 chimeric mice.These results indicate that, although host DCs disappear rapidly after allogeneic bone marrow transplantation, they prime donor T cells before their disappearance and play a critical role in triggering donor CD8 + T cell-mediated GVHD.