Dendrimer-modified Gold Nanorods as a Platform for Combinational Gene Therapy and Photothermal Therapy of Tumors

作者：Lili Ye, Yaoming Chen, Jizong Mao, Xiaotian Lei, Qian Yang, Chunhui Cui · 发表于：Research Square · 年份：2021 · DOI：10.21203/rs.3.rs-263824/v1 · 研究领域：RNA Interference and Gene Delivery、Advanced biosensing and bioanalysis techniques、Nanoplatforms for cancer theranostics

Abstract Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics. Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au NR@PAMAM-GX1) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection. Conclusion: The synthesized Au NR@PAMAM-GX1 is a potential nanoplatform for tumor imaging and treatment.