Genome wide comparative analysis of the BAHD superfamily in seven Rosaceae species and expression analysis in pear (Pyrus☓ bretschneideri)

作者：Chunxin Liu, Xin Qiao, Qionghou Li, Weiwei Zeng, Shuwei Wei, Xin Wang, Yangyang Chen, Angela Xiao Wu, Wu Jun, Hao Yin, Shaoling Zhang · 发表于：Research Square · 年份：2019 · DOI：10.21203/rs.2.13258/v1 · 被引用次数：1 · 研究领域：Plant biochemistry and biosynthesis、Plant Gene Expression Analysis、Plant Reproductive Biology

Abstract Background: The BAHD acyltransferase superfamily exhibits various biological roles in plants, including regulation the fruit quality; catalytic synthesis of terpene, phenolic and esters; improvement of stress resistance. However, the copy number, evolutionary history and potential functions of the BAHD superfamily genes in the genome sequenced Rosaceae species remains unclear. Results: Totally, 755 BAHD genes were obtained from the genomes of seven Rosaceae fruit species (Pyrus bretschneideri, Malus domestica, Prunus avium, Prunus persica, Fragaria vesca, Pyrus communis and Rubus occidentalis). Based on the classification results from model plants, we divided the BAHD family genes into seven subgroups (I-a, I-b, II-a, II-b, III-a, IV, V). Based on intra-species synteny analysis, 61 syntenic gene pairs were detected from the six Rosaceae species. Dispersed gene duplication occurred frequently in all investigated species. Different modes of duplicated gene pairs identified in each investigated species show that the Ka/Ks is less than one, indicating they evolved through purifying selection. Based on the correlation analysis between ester content and expression level of BAHD genes at different development stages, we selected five genes to perform qRT-PCR verification, and the results showed that Pbr020016.1, Pbr019034.1, Pbr014028.1 and Pbr029551.1 are the important candidate genes involved in aroma formation during pear fruit development. Conclusion: We have thoroughly...