Experimental study on nuclear-targeted plasmid-based short hairpin RNA (ShRNA) to hypoxia inducible factor-1 α (PshHIF-1 α ) PshHIF-1 α nano-drug carrier system for breast cancer treatment in rats

作者：Gang Sun, Jin Zhang, Shuai Wu, Yun Liu · 发表于：Materials Express · 年份：2021 · DOI：10.1166/mex.2021.2029 · 被引用次数：3 · 研究领域：RNA modifications and cancer、interferon and immune responses、Cancer, Hypoxia, and Metabolism

This study was aimed at exploring the regulatory mechanism of auclear-targeted pshHIF-1 α nano-drug carrier system (NPNCS) in the treatment of breast cancer in rats. MDA-MB-231 cell was cultured and DJ-1 and PTEN mRNA level was detected by qRT-PCR along with analysis of cell viability by CCK-8 and apoptosis by means of flow cytometry. The rats were assigned into control group, nuclear-targeted pshHIF-1 α nanopharmaceutical system treatment group, and nuclear-targeted pshHIF-1 α nano-pharmaceutical system+PTEN inhibitor SF1670 group, followed by analysis of cell proliferation by EdU staining, cell apoptosis by flow cytometry, and the levels of DJ-1, PTEN, and p-AKT. Compared with MDA-MB-231 cells treated with NPNCS, cells without intervention showed decreased PTEN and increased DJ-1 level. NPNCS induced cell apoptosis. Administration of nuclear-targeted pshHIF-1 α nano-drug delivery system down-regulated DJ-1 and up-regulated PTEN. Combined treatment with SF1670 promoted p-AKT level and decreased the inhibitory effect of NPNCS on p-AKT level. In conclusion, the nuclear-targeted pshHIF-1 α nano-drug delivery system down-regulated DJ-1 and exerted a tumor suppressor and pro-apoptotic effect, which wasrelated to p-AKT phosphorylation and up-regulation of PTEN.