Fungi Contribute More to N2O Emissions than Bacteria in Nitrogen Fertilized Lawn Soil

作者：Zhifeng Xun, Wansheng Yang, Liu Jun, Baihui Ren, Long Bai · 发表于：Authorea · 年份：2020 · DOI：10.22541/au.158162264.48999275 · 被引用次数：3 · 研究领域：Peatlands and Wetlands Ecology、Soil Carbon and Nitrogen Dynamics

Lawn is the primary vegetation utilized for urban sporting grounds and garden greenbelts. The N fertilizer addition induced higher N2O emission in various soils including the lawn soil, however, a key gap in knowledge lies in identifying the ecological consequences of the N2O emission and potentially associated microbial mechanisms. N2O is a product of microbial participation in nitrogen transformation processes, which is closely related to the use of nitrogen fertilizer. This study evaluated the effect of N fertilizer on N2O emissions and associated microbial mechanism in lawn soil through incubated experiment under different N application rates [300 kg ha yr-1 (N300), 225 kg ha yr-1 (N225), 150 kg ha yr-1 (N150) and control (N0)]. In addition, the contribution of different microbial communities to N2O emissions was quantified by combining biological inhibitors with high-throughput sequencing. The results indicated that N fertilizer addition induced higher N2O emissions in lawn soil, showed the highest in the N225 treatment. The contribution of fungi to N2O emissions was 45%, significantly higher than that of bacteria (31%). The dominant fungi in the lawn soil included Ascomycota, Basidiomycota, and Mucoromycota. N fertilizer significantly increased the relative abundance of Ascomycota and decreased the relative abundance of Basidiomycota. We found a positive correlation between N2O emission and Ascomycota through RDA analysis. The growth trend of Ascomycota during the four ...