Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomes

作者：Barry R. Lentz, Yechezekel Barenholz, Thomas Edward Thompson · 发表于：Biochemistry · 年份：1976 · DOI：10.1021/bi00665a029 · 被引用次数：386 · 研究领域：Lipid Membrane Structure and Behavior、Protein Structure and Dynamics、Spectroscopy and Quantum Chemical Studies

The fluorescence depolarization associated with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene is used to monitor the changes in fluidity accompanying the gel-liquid crystalline phase transition in synthetic phosphatidycholine dispersions. The parameters of the phase transition are determined for both large, multilamellar liposomes and small, single-lamellar vesicles. These parameters are compared with those obtained using other techniques. In addition, the data are interpreted in terms of two limiting molecular models, which in turn offer insight into the structural differences between multilammelar liposomes and small vesicles.