Enlightening the Taxonomy Darkness of Human Gut Microbiomes With Cultured Biobank

作者：Chang Liu, Meng‐Xuan Du, Rexiding Abuduaini, Haiying Yu, Dan‐Hua Li, Yujing Wang, Nan Zhou, Min-Zhi Jiang, Peng-Xia Niu, Shanshan Han, Hong‐He Chen, Wenyu Shi, Linhuan Wu, Yu‐Hua Xin, Juncai Ma, Yuguang Zhou, Cheng‐Ying Jiang, Hongwei Liu, Shuang‐Jiang Liu · 发表于：Research Square · 年份：2020 · DOI：10.21203/rs.3.rs-74101/v1 · 被引用次数：8 · 研究领域：Gut microbiota and health、Epigenetics and DNA Methylation

Abstract Background The cultivated gut microbial resource plays essential role in gut microbiome studies such as gut microbial function and their interactions with host. Though several major studies had been performed to understand the cultured human gut microbiota, up to 70% of the Unified Human Gastrointestinal Genome species remain uncultivated and their taxonomy is not clear. Large-scale gut microbial isolation and identification and their access to pubic are imperative for gut microbial studies and for understanding of the human gut microbial functions. Results Here, we report the construction of an human Gut Microbial Biobank (hGMB) (homepage: hgmb.nmdc.cn ) by large-scale cultivation of 10,558 isolates from 239 feces of healthy Chinese volunteers, and deposited 1,170 strains representing 404 different species in International Depository Authority for long-term preservation and public access worldwidely. We discovered and denominated 107 new species, and proposed 28 new genera and 3 new families. The new species and their newly sequenced genomes uncovered 16 “most-wanted” or “medium priority” taxa proposed by the Human Microbiome Project and 42 previously-uncultured MAGs in IGGdb, respectively. The hGMB represented over 80% of the common and dominant human gut microbial genera or species of global human gut 16S rRNA gene amplicon data (n=11,647), and covered 70% of the known genes (KEGG Orthologys) and 10% of the functionally-unknown genes in the global human gut gene c...