Phytochrome Radioimmunoassay

作者：Robert E. Hunt, Lee H. Pratt · 发表于：PLANT PHYSIOLOGY · 年份：1979 · DOI：10.1104/pp.64.2.327 · 被引用次数：50 · 研究领域：Light effects on plants

A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay and requires less than 1 microliter of crude extract from dark-grown plants for quantitation of phytochrome. Measurement of phytochrome in crude extracts by radioimmunoassay gives values about 25% greater than those obtained by spectral assay. The amount of phytochrome detected in crude extracts of light-grown oats by radioimmunoassay is approximately 1% of that detected in comparable extracts from dark-grown oats. General interference by crude plant extracts with radioimmunoassays was also observed and corrected for.