Identification of Flavin Mononucleotide as a Cell‐Active Artificial N 6 ‐Methyladenosine RNA Demethylase

作者：Li‐Jun Xie, Xiaoti Yang, Rui‐Li Wang, Hou‐Ping Cheng, Zhi‐Yan Li, Li Liu, Lanqun Mao, Ming Wang, Liang Cheng · 发表于：Angewandte Chemie · 年份：2019 · DOI：10.1002/ange.201900901 · 被引用次数：17 · 研究领域：RNA modifications and cancer、Epigenetics and DNA Methylation、Cancer-related gene regulation

Abstract N 6 ‐Methyladenosine (m 6 A) represents a common and highly dynamic modification in eukaryotic RNA that affects various cellular pathways. Natural dioxygenases such as FTO and ALKBH5 are enzymes that demethylate m 6 A residues in mRNA. Herein, the first identification of a small‐molecule modulator that functions as an artificial m 6 A demethylase is reported. Flavin mononucleotide (FMN), the metabolite produced by riboflavin kinase, mediates substantial photochemical demethylation of m 6 A residues of RNA in live cells. This study provides a new perspective to the understanding of demethylation of m 6 A residues in mRNA and sheds light on the development of powerful small molecules as RNA demethylases and new probes for use in RNA biology.