Production of Monoclonal Antibodies

作者：Wayne M. Yokoyama, Michelle Christensen, Gary Dos Santos, D M Miller · 发表于：Current Protocols in Immunology · 年份：2006 · DOI：10.1002/0471142735.im0205s74 · 被引用次数：54 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Protein purification and stability、Viral Infectious Diseases and Gene Expression in Insects

This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).