Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens

作者：Felix Kwame Amevor, Zhifu Cui, Xiaxia Du, Zifan Ning, Xun Deng, Dan Xu, Gang Shu, Youhao Wu, Xueqing Cao, Wei Shuo, Yaofu Tian, Diyan Li, Yan Wang, Yao Zhang, Xiaohui Du, Qing Zhu, Xue Han, Xiaoling Zhao · 发表于：Frontiers in Immunology · 年份：2022 · DOI：10.3389/fimmu.2022.860889 · 被引用次数：34 · 研究领域：Animal Nutrition and Physiology、Antioxidant Activity and Oxidative Stress、Ginger and Zingiberaceae research

In aged animals, the physiological functions of the gastrointestinal tract (GIT) are reduced. Dietary intervention is necessary to re-activate GIT functions. The objective of this study was to investigate the impacts of dietary combination of quercetin (Q) and vitamin E (VE) on the intestinal structure and barrier integrity in aged breeder chickens. A sum of 400 (65-wks-old) Tianfu breeder hens were randomly allotted into four (4) groups with four (4) replicates, and fed with basal diet; basal diet supplemented with 0.4g/kg of Q; basal diet supplemented with 0.2g/kg of VE; and basal diet supplemented with the combination of Q (0.4 g/kg) and VE (0.2 g/kg) for 14 weeks. At the end of the 14 th week, serum and gut segments were collected from eight hens per group for analyses. The results showed that Q+VE exerted synergistic effects on intestinal morphology by promoting villi height and crypt depth (P < 0.05), as well as mitigated the intestinal inflammatory damage of the aged hens, but decreased the concentration of serum D-lactate and diamine oxidase; and increased the levels of secretory immunoglobulin A (sIgA) and Mucin-2 mRNA (P < 0.05). Furthermore, the mRNA expression of intestinal tight junction proteins including occludin, ZO1 , and claudin-1 was increased by Q+VE (P < 0.05). Moreover, Q+VE decreased the mRNA expression of the pro-inflammatory genes ( TNF-α, IL-6 , and IL-1β ), and increased the expression of anti-inflammatory genes ( IL-10 and IL-4...