Wnt/β-Catenin Participates in the Repair of Acute Respiratory Distress Syndrome-Associated Early Pulmonary Fibrosis via Mesenchymal Stem Cell Microvesicles

作者：Xingcai Zhang, Lifang Ye, Wan Tang, Yiqin Ji, Zheng Li, Yijun Chen, Qidong Ge, Changshun Huang · 发表于：Dove Medical Press (Taylor and Francis Group) · 年份：2022 · 被引用次数：12 · 研究领域：Mesenchymal stem cell research、Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis、Neonatal Respiratory Health Research

Xingcai Zhang,1,* Lifang Ye,2,* Wan Tang,1 Yiqin Ji,1 Li Zheng,1 Yijun Chen,1 Qidong Ge,3 Changshun Huang1 1Department of Anesthesiology, Ningbo City First Hospital, Ningbo, Zhejiang, People’s Republic of China; 2Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, People’s Republic of China; 3Department of Breast Surgery, HuaMei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Changshun Huang; Qidong Ge, Tel +86-574-87085521, Fax +86-574-87085588, Email nbhcs1967@163.com; geqidong1986@126.comPurpose: The main aim of the present study was to establish whether mesenchymal stem cell microvesicles (MSC MVs) exert anti-fibrotic effects and investigate the mechanisms underlying these effects in a mouse model of acute respiratory distress syndrome (ARDS)-associated early pulmonary fibrosis.Methods: An ARDS-associated pulmonary fibrosis model was established in mice by an intratracheal injection of lipopolysaccharide (LPS). At 1, 3, and 7 days after LPS-mediated injury, the lungs of mice treated with MSC MVs and untreated controls were carefully excised and fibrosis was assessed based on the extent of collagen deposition. In addition, the development of epithelial–mesenchymal transition (EMT) was evaluated based on loss of E-cadherin and zona occludens-1 (ZO-1) along with ...