KLF16 Downregulates the Expression of Tumor Suppressor Gene TGFBR3 to Promote Bladder Cancer Proliferation and Migration
作者：Xiaosong Chen, Ping Wang, Tongwen Ou, Jin Li · 发表于：DOAJ (DOAJ: Directory of Open Access Journals) · 年份：2022 · 被引用次数：13 · 研究领域：Kruppel-like factors research、Cancer-related gene regulation、Genetic Syndromes and Imprinting
Xiaosong Chen,1,* Ping Wang,2,* Tongwen Ou,1 Jin Li1 1Department of Urology, Xuanwu Hospital of The Capital Medical University, Xuanwu Hospital, Beijing, Peopleâs Republic of China; 2Department of General Practice, Beijing Xicheng District White Paper Community Health Service Center, Beijing, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Xiaosong ChenDepartment of Urology, Xuanwu Hospital of The Capital Medical University, Xuanwu Hospital, No. 45, Changchun Street, Xicheng District, Beijing, 100053, Peopleâs Republic of China, Tel +86-10-83198899, Email cxsyzhjdsh@sohu.comIntroduction: Krüppel-like factors (KLFs), which comprise 17 family members, exert important functions during the development of cancer. The role of KLF16 seems controversial in carcinogenesis because both tumor suppressive and promoting effects have been reported.Methods: The expression level of KLF16 was analyzed based on public data sets from The Cancer Genome Atlas (TCGA) and evaluated by immunohistochemical (IHC) staining. CCK8 assay, colony formation analysis, transwell assays and the PI/Annexin V-APC assay kit were performed to detect cell growth, colony formation, cell migration and apoptosis of BC cells. Xenograft tumorigenesis assay was performed to detect the KLF16 expression on BC growth in vivo. Dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP)-qPCR assay were performed to analyze the inte...