Expression of anti-NbHK single-chain antibody in fusion with NSlmb enhances the resistance to Nosema bombycis in Sf9-III cells

作者：Renze Zhang, Shiyi Zheng, Hongyun Huang, Xi Sun, Yukang Huang, Junhong Wei, Guoqing Pan, Chunfeng Li, Zeyang Zhou · 发表于：Bulletin of Entomological Research · 年份：2022 · DOI：10.1017/s0007485321001036 · 被引用次数：7 · 研究领域：Invertebrate Immune Response Mechanisms、Viral Infectious Diseases and Gene Expression in Insects、Insect Resistance and Genetics

Abstract Nosema bombycis is a destructive and specific intracellular parasite of silkworm, which is extremely harmful to the silkworm industry. N. bombycis is considered as a quarantine pathogen of sericulture because of its long incubation period and horizontal and vertical transmission. Herein, two single-chain antibodies targeting N. bombycis hexokinase (NbHK) were cloned and expressed in fusion with the N-terminal of Slmb (a Drosophila melanogaster FBP), which contains the F-box domain. Western blotting demonstrated that Sf9-III cells expressed NSlmb–scFv-7A and NSlmb–scFv-6H, which recognized native NbHK. Subsequently, the NbHK was degraded by host ubiquitination system. When challenged with N. bombycis , the transfected Sf9-III cells exhibited better resistance relative to the controls, demonstrating that NbHK is a prospective target for parasite controls and this approach represents a potential solution for constructing N. bombycis -resistant Bombyx mori .