Seroprevalence of anti-SARS-CoV-2 antibodies in Thai adults during the first three epidemic waves

作者：Hatairat Lerdsamran, Anek Mungaomklang, Sopon Iamsirithaworn, Jarunee Prasertsopon, Witthawat Wiriyarat, Suthee Saritsiri, Ratikorn Anusorntanawat, Nirada Siriyakorn, Poj Intalapaporn, Somrak Sirikhetkon, Kantima Sangsiriwut, Worawat Dangsakul, Suteema Sawadpongpan, Nattakan Thinpan, Kuntida Kitidee, Pilailuk Akkapaiboon Okada, Ranida Techasuwanna, Noparat Mongkalangoon, Kriengkrai Prasert, Pilaipan Puthavathana · 发表于：PLoS ONE · 年份：2022 · DOI：10.1371/journal.pone.0263316 · 被引用次数：12 · 研究领域：SARS-CoV-2 and COVID-19 Research、COVID-19 Clinical Research Studies、COVID-19 epidemiological studies

This study determined the presence of anti-SARS-CoV-2 antibodies in 4964 individuals, comprising 300 coronavirus disease-19 (COVID-19) prepandemic serum samples, 142 COVID-19 patients, 2113 individuals at risk due to their occupations, 1856 individuals at risk due to sharing workplaces or communities with COVID-19 patients, and 553 Thai citizens returning after spending extended periods of time in countries with a high disease prevalence. We recruited participants between May 2020 and May 2021, which spanned the first two epidemic waves and part of the third wave of the COVID-19 outbreaks in Thailand. Their sera were tested in a microneutralization and a chemiluminescence immunoassay for IgG against the N protein. Furthermore, we performed an immunofluorescence assay to resolve discordant results between the two assays. None of the prepandemic sera contained anti-SARS-CoV-2 antibodies, while antibodies developed in 88% (15 of 17) of the COVID-19 patients at 8-14 days and in 94-100% of the patients between 15 and 60 days after disease onset. Neutralizing antibodies persisted for at least 8 months, longer than IgG antibodies. Of the 2113 individuals at risk due to their occupation, none of the health providers, airport officers, or public transport drivers were seropositive, while antibodies were present in 0.44% of entertainment workers. Among the 1856 individuals at risk due to sharing workplaces or communities with COVID-19 patients, seropositivity was present in 1.9, 1.5, a...