Reference Material 8376 microbial pathogen DNA standards for detection and identification

作者：Jason G. Kralj, Dieter M. Tourlousse, Monique E. Hunter, Erica L. Romsos, Blaza Toman, Peter M. Vallone, Scott A. Jackson · 年份：2022 · DOI：10.6028/nist.sp.260-225 · 被引用次数：9 · 研究领域：Molecular Biology Techniques and Applications、Salmonella and Campylobacter epidemiology、Genomics and Phylogenetic Studies

Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8 buffer. Each component consists of one skirted 0.5-mL tube containing approximately 100 uL of DNA solution. Each tube is labeled and sealed with a removable screwcap. Here, we have documented the production, analytical methods, and statistical evaluation of realizing this RM.