Untargeted serum metabolomics and potential biomarkers for Sjögren's syndrome

作者：Tingting Xu, Yunke Guo, Jiawei Lu, Jinjun Shan, Lili Lin, Wenjuan Qian, Wenjun Chen, Jue Wang, Xiangyu Lv, Mengying Ke, Deshun Kong, Qiuxiang Shen, Youjuan Zhu, Ping Liu, Jinfeng Su, Lu Wang, Yuhua Li, Pan Gao, Shijia Liu · 发表于：Clinical and Experimental Rheumatology · 年份：2021 · DOI：10.55563/clinexprheumatol/ylte6v · 被引用次数：30 · 研究领域：Salivary Gland Disorders and Functions、Growth Hormone and Insulin-like Growth Factors、Ginseng Biological Effects and Applications

Objective. At present, the pathogenesis of Sjgren's syndrome (SS) remains unclear. This research aimed to identify differential metabolites that contribute to SS diagnosis and discover the disturbed metabolic pathways. Methods. Recent advances in mass spectrometry have allowed the identification of hundreds of unique metabolic signatures and the exploration of altered metabolite profiles in disease. In this study, 505 candidates including healthy controls (HCs) and SS patients were recruited and the serum samples were collected. A non-targeted gas chromatography-mass spectrometry (GC-MS) serum metabolomics method was used to explore the changes in serum metabolites. Results. We found SS patients and HCs can be distinguished by 21 significant metabolites. The levels of alanine, tryptophan, glycolic acid, pelargonic acid, cis-1-2-dihydro-1-2-naphthalenediol, diglycerol, capric acid, turanose, behenic acid, dehydroabietic acid, stearic acid, linoleic acid, heptadecanoic acid, valine, and lactic acid were increased in serum samples from SS patients, whereas levels of catechol, anabasine, 3-6-anhydro-D-galactose, beta-gentiobiose, 2-ketoisocaproic acid and ethanolamine were decreased. The significantly changed pathways included the following: Linoleic acid metabolism; unsaturated fatty acid biosynthesis; aminoacyl-tRNA biosynthesis; valine, leucine, and isoleucine biosynthesis; glycerolipid metabolism; selenocompound metabolism; galactose metabolism; alanine, aspartate and glutama...