Enhancer transcription detected in the nascent transcriptomic landscape of bread wheat

作者：Yilin Xie, Yan Chen, Zijuan Li, Jiafu Zhu, Min Liu, Yijing Zhang, Zhicheng Dong · 发表于：Genome biology · 年份：2022 · DOI：10.1186/s13059-022-02675-1 · 被引用次数：43 · 研究领域：Genomics and Chromatin Dynamics、RNA and protein synthesis mechanisms、Genomics and Phylogenetic Studies

The precise spatiotemporal gene expression is orchestrated by enhancers that lack general sequence features and thus are difficult to be computationally identified. By nascent RNA sequencing combined with epigenome profiling, we detect active transcription of enhancers from the complex bread wheat genome. We find that genes associated with transcriptional enhancers are expressed at significantly higher levels, and enhancer RNA is more precise and robust in predicting enhancer activity compared to chromatin features. We demonstrate that sub-genome-biased enhancer transcription could drive sub-genome-biased gene expression. This study highlights enhancer transcription as a hallmark in regulating gene expression in wheat.