Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma

作者：Xiaoli Wu, Xiaoqing Meng, Tse-Shao Chang, Shuo Feng, Miki Lee, Sangeeta Jaiswal, Eun-Young Choi, Тран Дай Лам, Hui Jiang, Thomas D. Wang · 发表于：Photoacoustics · 年份：2022 · DOI：10.1016/j.pacs.2022.100355 · 被引用次数：9 · 研究领域：Proteoglycans and glycosaminoglycans research、Peptidase Inhibition and Analysis、Hepatocellular Carcinoma Treatment and Prognosis

Background: Hepatocellular carcinoma (HCC) is rising steadily in incidence, and more effective methods are needed for early cancer detection and image-guided surgery. Methods: We used a structural model to optimize the peptide sequence. Specific binding was validated in vitro with knockdown, competition, and co-localization assays. Multi-modal imaging was performed to validate specific binding in vivo in orthotopically-implanted human xenograft tumors. Results: . The target-to-background ratio was significantly higher for the target versus control for both modalities. Ex-vivo evaluation using human HCC specimens supported the ability of the peptide to distinguish HCC from other liver pathologies. Conclusions: We have identified a peptide specific for CD44 with properties that are promising for clinical translation to image HCC in vivo.