Injectable and Self-Healing Probiotics-Loaded Hydrogel for Promoting Superbacteria-Infected Wound Healing

作者：Li Mei, Dongjie Zhang, Huarong Shao, Yuanping Hao, Ting Zhang, Weiping Zheng, Yanjing Ji, Peixue Ling, Yun Lu, Qihui Zhou · 发表于：ACS Applied Materials & Interfaces · 年份：2022 · DOI：10.1021/acsami.1c23713 · 被引用次数：126 · 研究领域：Wound Healing and Treatments、Seaweed-derived Bioactive Compounds、Corneal Surgery and Treatments

Superbacteria-induced skin wound infections are huge health challenges, resulting in significant financial and medical costs due to notable morbidity and mortality worldwide. Probiotics are found in the skin and are effective in treating bacterial infection, moderating the microbial dysbiosis and inflammation induced by pathogens, regulating the immune system, as well as even promoting tissue repair. However, improving their colonization efficiency and viability remains a large obstacle for proper applications. Inspired by probiotic therapy and the natural extracellular matrix structure, hyaluronate-adipic dihydrazide/aldehyde-terminated Pluronic F127/fucoidan hydrogels loaded with Lactobacillus rhamnosus ( [email protected] ) with unique (bio)physicochemical characteristics were developed through the dynamic Schiff-base reaction for superbacteria-infected trauma management. The developed [email protected] exhibit a shortened gelation time, enhanced mechanical strength, and excellent self-healing and liquid-absorption abilities. Importantly, their anti-superbacteria ( Pseudomonas aeruginosa ) effect was greatly increased in a dose-dependent fashion. Additionally, in vitro evaluation shows that the prepared [email protected] containing appropriate probiotic concentrations (less than 1 × 10 7 CFU/mL) possess satisfactory cytocompatibility and blood compatibility. Further, compared to the HPF hydrogel, in vivo the hydrogel combined with probiotics significantly inhibits P. aerug...