Molecular Mechanism of the β-Lactamase Mediated β-Lactam Antibiotic Resistance of Pseudomonas aeruginosa Isolated From a Chinese Teaching Hospital

作者：Hailong Lin, Chunlin Feng, Tingting Zhu, Anqi Li, Shuang Liu, Lei Zhang, Qiaoling Li, Xueya Zhang, Li Lin, Junwan Lu, Xi Lin, Kewei Li, Hailin Zhang, Teng Xu, Changchong Li, Qiyu Bao · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.855961 · 被引用次数：10 · 研究领域：Antibiotic Resistance in Bacteria、Antibiotics Pharmacokinetics and Efficacy、Antibiotic Use and Resistance

Pseudomonas aeruginosa can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery. To investigate the molecular characteristics of β-lactam antibiotic resistance of P. aeruginosa isolated from a hospital population between 2015 and 2017, in this study, the antimicrobial susceptibility and the resistance gene profile of the bacteria were determined. The Pulsed-field gel electrophoresis (PFGE) was used to characterize the clonal relatedness and sequencing and comparative genomic analysis were performed to analyze the structure of the resistance gene-related sequences. As a result, of the 260 P . aeruginosa strains analyzed, the resistance rates for 6 β-lactam antibiotics ranged from 4.6 to 9.6%. A total of 7 genotypes of 44 β-lactamase genes were identified in 23 isolates (8.9%, 23/260). Four transconjugants from different donors carrying bla CARB-3 exhibited a phenotype of reduced susceptibility to piperacillin–tazobactam, ceftazidime, and cefepime, and 2 transconjugants harboring bla IMP-45 exhibited a phenotype of reduced susceptibility to carbapenems. bla CARB positive isolates ( n = 12) presented six PFGE patterns, designated groups A to F. Two bla genes ( bla IMP-45 and bla OXA-1 ) in PA1609 related to a class 1 integron ( intI1 - bla IMP-45- bla OXA-1 - aac(6′)-Ib7 - catB3 - qacE∆1 - sul1 ) were encoded on a plasmid (pPA1609-475), while the bla CARB-3 gene of PA1616 also related to a class 1 integron was located on the chromosome. The ...