How fluorescent labels affect the kinetics of the toehold-mediated DNA strand displacement reaction

作者：Chengxu Li, Zhigang Li, Wenjie Han, Xue Yin, Xiaoyu Liu, Shiyan Xiao, Haojun Liang · 发表于：Chemical Communications · 年份：2022 · DOI：10.1039/d2cc01072k · 被引用次数：12 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、RNA Interference and Gene Delivery

End modification of the toehold domain or near to it using fluorophore dyes or quenchers can significantly modulate the kinetics of the toehold-mediated strand displacement reaction (TMSDR) by almost two orders of magnitude. The labels at the end of the signal strand impede the TMSDR, while those at the end of the toehold domain of the substrate strand accelerate the TMSDR kinetics.