Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

作者：Kai Shi, Qian Zhao, Minghui Shao, Ying Duan, Dongfeng Li, Yangqing Lu, Yanfei Tang, Chungang Feng · 发表于：Metabolites · 年份：2022 · DOI：10.3390/metabo12050367 · 被引用次数：40 · 研究领域：Animal Nutrition and Physiology、Metabolomics and Mass Spectrometry Studies、Meat and Animal Product Quality

The selection for improved body weight is an effective approach in animal breeding. Guangxi Partridge chickens have differentiated into two lines under selective breeding, which include line S and line D that have shown statistically significant differences in body weight. However, the meat quality analysis in our study indicated that the quality of breast and thigh muscles in line S chickens changed, which included increased values of L*, b*, and drip loss and decreased a* value, pH, and shear force in skeletal muscles. To illuminate the effect of selection on skeletal muscles, LC-MS/MS metabolomics was performed to explore differentiated metabolites in divergent tissues from the two chicken lines. The results of principal component analysis and orthogonal projection to latent structures discriminant analysis suggested that metabolites of different groups were separated, which suggested that selective breeding certainly affected metabolism of skeletal muscles. KEGG analysis identified that valine, leucine, and isoleucine biosynthesis, glycerophospholipid metabolism, and glutathione metabolism noteworthily changed in breast muscle. Amino sugars and nucleotide sugar metabolism, ascorbate and aldarate metabolism, the pentose phosphate pathway, pentose and glucuronate interconversions, fructose and mannose metabolism, and glycerophospholipid metabolism were remarkedly identified in thigh muscle. These screened pathways suggested oxidative stress in breast and thigh muscles, whic...