Shape-Recoverable Hyaluronic Acid–Waterborne Polyurethane Hybrid Cryogel Accelerates Hemostasis and Wound Healing

作者：Min Wang, Juanjuan Hu, Yangcen Ou, Xueling He, Yanjun Wang, Chen‐Yu Zou, Yanlin Jiang, Feng Luo, Dan Lu, Zhen Li, Jiehua Li, Hong Tan · 发表于：ACS Applied Materials & Interfaces · 年份：2022 · DOI：10.1021/acsami.2c01310 · 被引用次数：77 · 研究领域：Wound Healing and Treatments、Surgical Sutures and Adhesives、Electrospun Nanofibers in Biomedical Applications

Wound dressings that promote quick hemostasis and are highly efficient in healing wounds are urgently needed to meet the increase in clinical demands worldwide. Herein, a dihydrazide-modified waterborne biodegradable polyurethane emulsion (PU-ADH) and oxidized hyaluronic acid (OHA) were autonomously cross-linked to form a hybrid hyaluronic acid–polyurethane (HA-PU) cryogel by hydrazone bonding at −20 °C. Through its specific macroporous structure (which is approximately 220 μm) constructed by aggregated PU-ADH particles and long-chain OHA, a dried cryogel can have a dramatically compressed volume (1/7 of its original volume) with stable fixation, and it can swell rapidly by absorbing water or blood to approximately 22 and 16 times its dried weight, respectively, in a few minutes. This instantaneous shape-recovering ability favors fast hemostasis in minimally invasive surgery. Moreover, this cryogel is superior to gauze, has excellent biocompatibility, and quickly coagulates blood (in approximately 2 min) by activating the endogenous coagulation system. Comparably, an injectable HA-PU hydrogel with the same components as the HA-PU cryogel was prepared at room temperature, and it exhibited good self-healing properties. An in vivo evaluation of a rat liver hemostasis model and rat skin defect model revealed that the cryogel in fast hemostasis has great potential and superior wound-healing abilities, decreases immune inflammation, and promotes the regeneration of angiogenesis and...