Cilo-seq: highly sensitive cell-in-library-out single-cell transcriptome sequencing with digital microfluidics

作者：Qianqian Zhang, Xing Xu, Li Lin, Jian Yang, Na Xing, Xin Chen, Lingling Wu, Jia Song, Chaoyong Yang · 发表于：Lab on a Chip · 年份：2022 · DOI：10.1039/d2lc00167e · 被引用次数：23 · 研究领域：Single-cell and spatial transcriptomics、Electrowetting and Microfluidic Technologies、Microfluidic and Bio-sensing Technologies

We described Cilo-seq for high-performance scRNA-seq library construction in a single device with programmed and addressable droplet handling based on digital microfluidics, which allowed simultaneously convenient single-cell isolation, efficient nucleic acid amplification, low-loss nucleic acid purification and high-quality library preparation.