Target-Binding Accelerated Response for Sensitive Detection of Basal H 2 O 2 in Tumor Cells and Tissues via a Dual-Functional Fluorescence Probe

作者：Mingxiu Wang, Feng Zhang, Caiqi Wang, Nan Yin, Yuting Wang, Guixin Qin, Qingling Xu, Jianhua Gong, Huizhen Liu, Xinrui Duan · 发表于：Analytical Chemistry · 年份：2022 · DOI：10.1021/acs.analchem.2c00400 · 被引用次数：55 · 研究领域：Molecular Sensors and Ion Detection、Sulfur Compounds in Biology、Redox biology and oxidative stress

Aberrant production of H 2 O 2 is involved in cancer. The levels of H 2 O 2 are significantly higher in tumor cells than in normal cells. It is important to develop fluorescent probes to image basal H 2 O 2 selectively in tumor cells. So far, a cancer cell-targeting probe to image basal H 2 O 2 has not been reported. Thus, we developed a fluorescent probe, BBHP, which contains benzil as a H 2 O 2 -recognition site and biotin as a target binding motif for the selective and sufficient detection of H 2 O 2 in tumor cells. BBHP enables a selective fluorescence turn-on response to H 2 O 2 . The binding of the probe with biotin receptors can greatly accelerate the fluorescence response to H 2 O 2 . As a result, BBHP can sufficiently image basal H 2 O 2 in biotin receptor-positive cancer cells and tumor tissues. Finally, BBHP was successfully applied to discriminate between cancerous and normal tissues.