Ginsenoside Rb1 Improves Metabolic Disorder in High-Fat Diet-Induced Obese Mice Associated With Modulation of Gut Microbiota

作者：Hong Yan Zou, Man Zhang, Xiaoting Zhu, Liyan Zhu, Shuo Chen, Mingjing Luo, Qinglian Xie, Yue Chen, Kangxi Zhang, Qingyun Bu, Yuchen Wei, Ye Tao, Qiang Li, Xing Yan, Zhihua Zhou, Chen Yang, Yu Li, Haokui Zhou, Chenhong Zhang, Xiaoyan You, Guangyong Zheng, Guoping Zhao · 发表于：Frontiers in Microbiology · 年份：2022 · 被引用次数：38 · 研究领域：Ginseng Biological Effects and Applications、Gut microbiota and health、Cancer-related molecular mechanisms research

Gut microbiota plays an important role in metabolic homeostasis. Previous studies demonstrated that ginsenoside Rb1 might improve obesity-induced metabolic disorders through regulating glucose and lipid metabolism in the liver and adipose tissues. Due to low bioavailability and enrichment in the intestinal tract of Rb1, we hypothesized that modulation of the gut microbiota might account for its pharmacological effects as well. Here, we show that oral administration of Rb1 significantly decreased serum LDL-c, TG, insulin, and insulin resistance index (HOMA-IR) in mice with a high-fat diet (HFD). Dynamic profiling of the gut microbiota showed that this metabolic improvement was accompanied by restoring of relative abundance of some key bacterial genera. In addition, the free fatty acids profiles in feces were significantly different between the HFD-fed mice with or without Rb1. The content of eight long-chain fatty acids (LCFAs) was significantly increased in mice with Rb1, which was positively correlated with the increase of Akkermansia and Parasuttereller , and negatively correlated with the decrease of Oscillibacter and Intestinimonas . Among these eight increased LCFAs, eicosapentaenoic acid (EPA), octadecenoic acids, and myristic acid were positively correlated with metabolic improvement. Furthermore, the colonic expression of the free fatty acid receptors 4 ( Ffar4 ) gene was significantly upregulated after Rb1 treatment, in response to a notable increase of LCFA in feces...