Photoacoustic microscopy of vascular adaptation and tissue oxygen metabolism during cutaneous wound healing

作者：Naidi Sun, Anthony C. Bruce, Bo Ning, Rui Cao, Yiming Wang, Fenghe Zhong, Shayn M. Peirce, Song Hu · 发表于：Biomedical Optics Express · 年份：2022 · DOI：10.1364/boe.456198 · 被引用次数：29 · 研究领域：Photoacoustic and Ultrasonic Imaging、Wound Healing and Treatments、Diabetic Foot Ulcer Assessment and Management

Cutaneous wounds affect millions of people every year. Vascularization and blood oxygen delivery are critical bottlenecks in wound healing, and understanding the spatiotemporal dynamics of these processes may lead to more effective therapeutic strategies to accelerate wound healing. In this work, we applied multi-parametric photoacoustic microscopy (PAM) to study vascular adaptation and the associated changes in blood oxygen delivery and tissue oxygen metabolism throughout the hemostasis, inflammatory, proliferation, and early remodeling phases of wound healing in mice with skin puncture wounds. Multifaceted changes in the vascular structure, function, and tissue oxygen metabolism were observed during the 14-day monitoring of wound healing. On the entire wound area, significant elevations of the arterial blood flow and tissue oxygen metabolism were observed right after wounding and remained well above the baseline over the 14-day period. On the healing front, biphasic changes in the vascular density and blood flow were observed, both of which peaked on day 1, remained elevated in the first week, and returned to the baselines by day 14. Along with the wound closure and thickening, tissue oxygen metabolism in the healing front remained elevated even after structural and functional changes in the vasculature were stabilized. On the newly formed tissue, significantly higher blood oxygenation, flow, and tissue metabolism were observed compared to those before wounding. Blood oxyge...