Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods

作者：Beliz Yüksektepe, Özlem Sefer, Gülin İnci Varol, Tuğba Teker, Mehmet Arslan, Büşra Nur Çetin, Figen Mert, Emre Yörük, Gülruh Albayrak · 发表于：European Journal of Biology · 年份：2022 · DOI：10.26650/eurjbiol.2022.1078448 · 被引用次数：3 · 研究领域：Mycotoxins in Agriculture and Food、Plant Pathogens and Fungal Diseases、Microbial infections and disease research

Objective: Fusarium spp. cause Fusarium head blight (FHB) and crown rot (CR) diseases. They also have harmful effects on animal and human health through their mycotoxins. Within the scope of this study, F. graminearum and F. culmorum isolates were purified from wheat ears and stalks contaminated with phytopathogens, which had been collected from various regions of Turkey, were identified and characterized by conventional and molecular methods. Materials and Methods: Sixty-eight Fusarium samples were isolated by single spore analysis and classified according to their macroconidia shape and size. Morphologically characterized samples were verified by amplification of SCAR markers. Their mating types (MAT) and chemotypes were also determined through polymerase chain reaction (PCR). Results: Thirty-eight F. graminearum and 30 F. culmorum isolates were identified via amplification of UBC85 and OPT18 SCAR markers, respectively. All isolates were determined as trichothecene producers by amplification of the tri5 gene. All F. graminarum isolates carry both MAT-1 and MAT-2 loci, whereas 7 of F. culmorum isolates were also determined as MAT-1 and 23 of them as MAT-2 mating types. Deoxynivalenol production capacity of all isolates was identified by tri13 amplification for chemotype determination. Conclusion: Routine monitoring of phytopathogens and their mycotoxin levels is a requirement since their annual levels may vary depending on environmental factors. This work provides knowledge ...