Electrostatic Charge‐Mediated Apoptotic Vesicle Biodistribution Attenuates Sepsis by Switching Neutrophil NETosis to Apoptosis

作者：Qianmin Ou, Lingping Tan, Yiting Shao, Fangcao Lei, Weiying Huang, Ning Yang, Yan Qu, Zeyuan Cao, Luhan Niu, Yao Liu, Xiaoxing Kou, Songtao Shi · 发表于：Small · 年份：2022 · DOI：10.1002/smll.202200306 · 被引用次数：71 · 研究领域：Neutrophil, Myeloperoxidase and Oxidative Mechanisms、Phagocytosis and Immune Regulation、Immune cells in cancer

Mesenchymal stem cell (MSC) therapy can attenuate organ damage and reduce mortality in sepsis; however, the detailed mechanism is not fully elucidated. In this study, it is shown that MSC-derived apoptotic vesicles (apoVs) can ameliorate multiple organ dysfunction and improve survival in septic mice. Mechanistically, it is found that tail vein-infused apoVs mainly accumulate in the bone marrow of septic mice via electrostatic charge interactions with positively charged neutrophil extracellular traps (NETs). Moreover, apoVs switch neutrophils NETosis to apoptosis via the apoV-Fas ligand (FasL)-activated Fas pathway. In summary, these findings uncover a previously unknown role of apoVs in sepsis treatment and an electrostatic charge-directed target therapeutic mechanism, suggesting that cell death is associated with disease development and therapy.