Single-Cell Transcriptome Integration Analysis Reveals the Correlation Between Mesenchymal Stromal Cells and Fibroblasts

作者：Chuiqin Fan, Maochuan Liao, Lichun Xie, Liangping Huang, Siyu Lv, Siyu Cai, Xing Su, Yue Wang, Hongwu Wang, Manna Wang, Yulin Liu, Yulin Liu, Yu Wang, Huijie Guo, Hanhua Yang, Yufeng Liu, Yufeng Liu, Tianyou Wang, Lian Ma · 发表于：Frontiers in Genetics · 年份：2022 · DOI：10.3389/fgene.2022.798331 · 被引用次数：36 · 研究领域：Mesenchymal stem cell research、Cancer Cells and Metastasis、Cancer-related molecular mechanisms research

Background: Mesenchymal stromal cells (MSCs) and fibroblasts show similar morphology, surface marker expression, and proliferation, differentiation, and immunomodulatory capacities. These similarities not only blur their cell identities but also limit their application. Methods: We performed single-cell transcriptome sequencing of the human umbilical cord and foreskin MSCs (HuMSCs and FSMSCs) and extracted the single-cell transcriptome data of the bone marrow and adipose MSCs (BMSCs and ADMSCs) from the Gene Expression Omnibus (GEO) database. Then, we performed quality control, batch effect correction, integration, and clustering analysis of the integrated single-cell transcriptome data from the HuMSCs, FMSCs, BMSCs, and ADMSCs. The cell subsets were annotated based on the surface marker phenotypes for the MSCs ( CD105 + , CD90 + , CD73 + , CD45 − , CD34 − , CD19 − , HLA-DRA − , and CD11b − ), fibroblasts ( VIM + , PECAM1 − , CD34 − , CD45 − , EPCAM − , and MYH11 − ), and pericytes ( CD146 + , PDGFRB + , PECAM1 − , CD34 − , and CD45 − ). The expression levels of common fibroblast markers ( ACTA2 , FAP , PDGFRA , PDGFRB , S100A4 , FN1 , COL1A1 , POSTN , DCN , COL1A2 , FBLN2 , COL1A2 , DES , and CDH11 ) were also analyzed in all cell subsets. Finally, the gene expression profiles, differentiation status, and the enrichment status of various gene sets and regulons were compared between the cell subsets. Results: We demonstrated 15 distinct cell subsets in the integrated single-c...